Guide to non-MVC plasmid construction

Viral vector submitted Genetic elements
AAV vectors ITR, promoter(s), transgene(s), polyA signal, any other genetic elements
Lentiviral vectors 5’LTR, status of 3’LTR (deleted of all enhancer elements or not), cPPT, promoter(s), transgene(s), WPRE
Retroviral vectors 5’LTR, status of 3’LTR (deleted of all enhancer elements or not), promoter(s), transgene(s), WPRE

If possible, we recommend you include a fluorescent reporter protein in your plasmid. This will help us troubleshoot any problems that may arise during virus production, and will enable you to gauge the efficiency of transduction in your experiments.

Revised on 2013-06-03 13:17:17 UTC